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About Myself
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Exaggerating here, but—world’s unfair!
X-inactivation (XI) analysis using the well-characterized CpG methylation site and polymorphic CAG repeats within the Androgen Receptor (AR) locus was performed using standard protocol (Allen et al., 1992). Digestion with the methylation-sensitive HpaII restriction enzyme followed by PCR amplification was used to determine the ratio of methylation status between the maternal and paternal X chromosome, parental samples were utilized to delineate allele status, the R1115H substitution was introduced into pENTR-KDM5C (human) (Iwase et al., 2007) using a PCR-based targeted mutagenesis. WT- and mutant KDM5C cDNA were then moved by LR recombination to a modified pHAGE, a lentivirus compatible mammalian expression plasmid (Murphy et al., 2006).After that little fiasco, I decide to chill at the Iwase Seaside Park. It’s beautiful there, with the ocean waves crashin’ and the sun settin’. I sit on a bench, tryin’ to calm down. But then, I see this group of kids playin’ soccer. They’re havin’ a blast, laughin’ and shoutin’. I can’t help but smile. It’s like their joy is contagious, ya know?
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Company History Since its founding in , Iwase Cosfa has been working to provide a richer, healthier, and more comfortable lifestyle for all. We aim to be a research, planning, and development proposal style trading company. Please check here before visiting.
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