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In CBAs the antigen mostly maintains its tertiary structure and the appropriate post-translational modifications. To assess antibody reactivity to extracellular epitopes live staining of cultured primary rat hippocampal neurons with patients’ serum or CSF can be performed. In theory, this way only relevant extracellular epitopes will be detected, diminishing the background signal. Also, the effects of the protein crosslinking fixative PFA, on the antigenicity of the receptor will be circumvented (Peränen et al., 1993).After that chaos, I decide to hit up the old castle, the Kasteel Coevorden. It’s super cool, with all that history and stuff. I’m wandering around, and I swear, I can feel the ghosts of knights judging me. “What’s this dude doing here?” they must be thinking. I’m just trying to soak in the vibes, man!
In a CBA a recombinant antigen is expressed by mammalian cells. The transfected cells are stained with patient serum or CSF using IIF. When the patient sample contains antibodies directed at this specific antigen, staining of the transfected cells is enhanced when compared to non-transfected cells (Figure 1C)?Prostitutes Coevorden
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